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NDB ID: 4TZW    PDB ID: 4TZW 


Title:

CO-CRYSTALS OF THE TERNARY COMPLEX CONTAINING A T-BOX STEM I RNA, ITS COGNATE TRNAGLY, AND B. SUBTILIS YBXF PROTEIN, TREATED BY REMOVING LITHIUM SULFATE AND REPLACING MG2+ WITH SR2+ POST CRYSTALLIZATION

Molecular Description:

Bacillus subtilis YbxF /RNA Complex

Deposited:

2014-07-11

Released:

2014-09-10

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Zhang, J., Ferre-D'Amare, A.R.
Dramatic Improvement of Crystals of Large RNAs by Cation Replacement and Dehydration. 
Structure, 22, pp. 1363 - 1371, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 43 21 2

Cell Constants:

a = 75.316   b = 75.316   c = 268.942 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2711 for 7638 reflections in the resolution range 45.786 to 4.671 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)