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NDB ID: 4WJ3    PDB ID: 4WJ3 


Title:

CRYSTAL STRUCTURE OF THE ASPARAGINE TRANSAMIDOSOME FROM PSEUDOMONAS AERUGINOSA

Molecular Description:

Glutamyl-tRNA(Gln) amidotransferase subunit A
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Glutamyl-tRNA(Gln) amidotransferase subunit C
Aspartate--tRNA(Asp/Asn) ligase/76mer-tRNA Complex

Deposited:

2014-09-29

Released:

2014-12-31

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 4 protein sequences

Primary Citation:

Suzuki, T., Nakamura, A., Kato, K., Soll, D., Tanaka, I., Sheppard, K., Yao, M.
Structure of the Pseudomonas aeruginosa transamidosome reveals unique aspects of bacterial tRNA-dependent asparagine biosynthesis 
Proc.Natl.Acad.Sci.USA, 112, pp. 382 - 387, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 93.14   b = 185.675   c = 290.359 (Ångstroms)

α = 90.0   β = 92.98   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2941 for 96481 reflections in the resolution range 46.769 to 3.705 Ångstroms with Fobs >  1.38 sigma(Fobs) and with I >  0.0 sigma(I)