## NDB ID: 4X23 PDB ID: 4X23 NAKB: 4X23

### Title:

CRYSTAL STRUCTURE OF CENP-C IN COMPLEX WITH THE NUCLEOSOME CORE PARTICLE

### Molecular Description:

HISTONE H3/CENP-A

HISTONE H4

HISTONE H2A

HISTONE H2B

VAL-ARG-ARG-SER-ASN-ARG-ILE-ARG-LEU-LYS-PRO-LEU-GLU-TYR-TRP-ARG-GLY-GLU-ARG-ILE-ASP-TYR/DNA Complex

### Deposited:

2014-11-25

### Released:

2014-12-10

### Structural Keywords:

B DOUBLE HELIX

### Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Chain I,S

(DA)(DT)(DC)(DG)(DA)(DG)(DA)(DA)(DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA) (DG)(DG)(

DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG) (DA)(DC)(DA)(DG)(D

C)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC)(DG)(DC)(DT)(DT)(DA)(DA) (DA)(DC)(DG)(DC)(DA)(DC)(DG

)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)(DC) (DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)

(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG) (DG)(DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC)(DC)(

DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA) (DG)(DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DC)(DA)(DG)(DA)(D

T)(DA)(DT)(DA)(DT)(DA)(DC) (DA)(DT)(DC)(DC)(DG)(DA)(DT)

Chain J,T

(DA)(DT)(DC)(DG)(DG)(DA)(DT)(DG)(DT)(DA)(DT)(DA)(DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC) (DA)(DC)(

DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC)(DT)(DA)(DG)(DG)(DG)(DA) (DG)(DT)(DA)(DA)(D

T)(DC)(DC)(DC)(DC)(DT)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DT)(DA)(DA) (DA)(DA)(DC)(DG)(DC)(DG)(DG

)(DG)(DG)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)(DA) (DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)

(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA) (DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(

DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG) (DA)(DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(D

C)(DC)(DG)(DG)(DG)(DA)(DT) (DT)(DC)(DT)(DC)(DG)(DA)(DT)

### Protein Sequence:

Click to show/hide 5 protein sequences

Chain A,E,K,O

RYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTI MPKDIQLAR

RIRGIEGGL

Chain C,G,M,Q

SRSNRAGLQFPVGRIHRLLRKGNYAERVGAGAPVYLAAVMEYLAAEVLELAGNAARDNKKTRIIPRHLQLAIRNDEELNK LLSGVTIAQ

GGVLPNIQAVLLP

Chain B,F,L,P

DNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG

Chain V,U,X,W

PNVRRSNRIRLKPLEYWRGERIDYQ

Chain D,H,N,R

ESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEG TKAVTKYTS

S

### Primary Citation:

Kato, H., Jiang, J.S., Zhou, B.R., Rozendaal, M., Feng, H., Ghirlando, R., Xiao, T.S., Straight, A.F., Bai, Y.

A conserved mechanism for centromeric nucleosome recognition by centromere protein CENP-C.

*Science*, **340**, pp. 1110 - 1113, 2013.

### Experimental Information:

X-RAY DIFFRACTION

### Space Group:

P 21 21 21

### Cell Constants:

a = 102.994 b = 176.102 c = 208.846 (Ångstroms)

α = 90.0 β = 90.0 γ = 90.0 (degrees)

### Refinement:

The structure was refined using the PHENIX program.
The R value is 0.2382 for 48623 reflections in
the resolution range 49.543 to 3.5 Ångstroms
with Fobs > 1.33 sigma(Fobs) and with I > 0.0 sigma(I)