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NDB ID: 4XLS    PDB ID: 4XLS 


Title:

CRYSTAL STRUCTURE OF T. AQUATICUS TRANSCRIPTION INITIATION COMPLEX WITH CARD CONTAINING UPSTREAM FORK PROMOTER.

Molecular Description:

DNA directed RNA polymerase subunit alpha
DNA directed RNA polymerase subunit beta
DNA directed RNA polymerase subunit beta'
DNA directed RNA polymerase subunit omega
DNA directed RNA polymerase sigma factor SigA
RNA polymerase-binding transcription factor CarD/DNA Complex

Deposited:

2015-01-13

Released:

2015-09-23

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 6 protein sequences

Primary Citation:

Bae, B., Chen, J., Davis, E., Leon, K., Darst, S.A., Campbell, E.A.
CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex. 
Elife, 4, pp. - , 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 43 21 2

Cell Constants:

a = 293.15   b = 293.15   c = 539.13 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.234 for 148127 reflections in the resolution range 49.79 to 4.01 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)