NDB ID: 4XLS PDB ID: 4XLS 
Title:
CRYSTAL STRUCTURE OF T. AQUATICUS TRANSCRIPTION INITIATION COMPLEX WITH CARD CONTAINING UPSTREAM FORK PROMOTER.
Molecular Description:
DNA directed RNA polymerase subunit alpha
DNA directed RNA polymerase subunit beta
DNA directed RNA polymerase subunit beta'
DNA directed RNA polymerase subunit omega
DNA directed RNA polymerase sigma factor SigA
RNA polymerase-binding transcription factor CarD/DNA Complex
Deposited:
2015-01-13
Released:
2015-09-23
Structural Keywords:
B DOUBLE HELIX, OVERHANGING BASES
Nucleic Acid Sequence:
Click to show/hide 2 nucleic acid sequences
Chain O,R
(DC)(DT)(DT)(DG)(DA)(DC)(DA)(DA)(DA)(DA)(DG)(DT)(DG)(DT)(DT)(DA)(DA)(DA)(DT)(DT) (DG)(DT)(
DG)(DC)(DT)(DA)(DT)(DA)(DC)(DT)
Chain P,S
(DG)(DC)(DA)(DC)(DA)(DA)(DT)(DT)(DT)(DA)(DA)(DC)(DA)(DC)(DT)(DT)(DT)(DT)(DG)(DT) (DC)(DA)(
DA)(DG)
Protein Sequence:
Click to show/hide 6 protein sequences
Chain A,B,G,H
MLESKLKAPVFTATTQGDHYGEFVLEPLERGFGVTLGNPLRRILLSSIPGTAVTSVYIEDVLHEFSTIPGVKEDVVEIIL NLKELVVRF
LDPKMASTTLILRAEGPKEVRAGDFTPSADVEIMNPDLHIATLEEGGKLYMEVRVDRGVGYVPAERHGIKD RINAIPVDAIFSPVRRVA
FQVEDTRLGQRTDLDKLTLRIWTDGSVTPLEALNQAVAILKEHLNYFANPEASLLPTPEVSK GEKRESAEEDLDLPLEELGLSTRVLHS
LKEEGIESVRALLALNLKDLRNIPGIGERSLEEIRQALAKKGFTLKE
Chain D,J
MKKEVRKVRIALASPEKIRSWSYGEVEKPETINYRTLKPERDGLFDERIFGPIKDYECACGKYKRQRFEGKVCERCGVEV TRSIVRRYR
MGHIELATPAAHIWFVKDVPSKIGTLLDLSATELEQVLYFNKYIVLDPKGAVLDGVPVEKRQLLTDEEYRE LRYGKQETYPLPAGVDAL
VKDGEEVVKGQELAPGVVSRMDGVALYRFPRRVRVDYLRKERAALRIPLSAWVEKEAYRPGE VLAELSEPYLFRAEESGVVELKDLAEG
HLIYLRQEEEVVARYFLPAGMTPLVVEGEIVEVGQPLAEGKGLLRLPRHMTAK EVEAEEEGDSVHLTLFLEWTEPKDYKVAPHMNVIVP
EGAKVQAGEKIVAAIDPEEEVIAEAEGVVHLHEPASILVVKARV YPFEDDVEVTTGDRVAPGDVLADGGKVKSEIYGRVEVDLVRNVVR
VVESYDIDARMGAEAIQELLKELDLEKLERELLEE MKHPSRARRAKARKRLEVVRAFLDSGNRPEWMILEAVPVLPPDLRPMVQVDGGR
FATSDLNDLYRRLINRNNRLKKLLAQ GAPEIIIRNEKRMLQEAVDAVIDNGRRGSPVTNPGSERPLRSLTDILSGKQGRFRQNLLGKRV
DYSGRSVIVVGPQLKLH QCGLPKRMALELFKPFLLKKMEEKAFAPNVKAARRMLERQRDIKDEVWDALEEVIHGKVVLLNRAPTLHRLG
IQAFQPVL VEGQSIQLHPLVCEAFNADFDGDQMAVHVPLSSFAQAEARIQMLSAHNLLSPASGEPLAKPSRDIILGLYYITQVRKEKK
GAGMAFATPEEALAAYERGEVALNAPIVVAGRETSVGRLKFVFANPDEALLAVAHGLLDLQDVVTVRYLGRRLETSPGRI LFARIVGEA
VGDEKVAQELIQMDVPQEKNSLKDLVYQAFLRLGMEKTARLLDALKYYGFTLSTTSGITIGIDDAVIPEEK QRYLEEADRKLRQIEQAY
EMGFLTDRERYDQVIQLWTETTEKVTQAVFKNFEENYPFNPLYVMAQSGARGNPQQIRQLCG MRGLMQKPSGETFEVPVRSSFREGLTV
LEYFISSHGARKGGADTALRTADSGYLTRKLVDVAHEIVVREADCGTTNYISV PLFQMDEVTRTLRLRKRSDIESGLYGRVLAREVEAL
GRRLEEGRYLSLEDVHFLIKAAEAGEVREVPVRSPLTCQTRYGV CQKCYGYDLSMARPVSIGEAVGVVAAESIGEPGTQLTMRTFHTGG
VAVGTDITQGLPRVIELFEARRPKAKAVISEIDGV VRIEEGEDRLSVFVESEGFSKEYKLPKDARLLVKDGDYVEAGQPLTRGAIDPHQ
LLEAKGPEAVERYLVDEIQKVYRAQG VKLHDKHIEIVVRQMLKYVEVTDPGDSRLLEGQVLEKWDVEALNERLIAEGKVPVAWKPLLMG
VTKSALSTKSWLSAASF QNTTHVLTEAAIAGKKDELIGLKENVILGRLIPAGTGSDFVRFTQVVDQRTLKAIEEARKEAVEAKEKEAPR
RPVRREQP GKGL
Chain C,I
MEIKRFGRIREVIPLPPLTEIQVESYKKALQADVPPEKRENVGIQAAFKETFPIEEGDKGKGGLVLDFLEYRIGDPPFSQ DECREKDLT
YQAPLYARLQLIHKDTGLIKEDEVFLGHLPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPDPARPGRYIA SIIPLPKRGPWIDLEVEA
SGVVTMKVNKRKFPLVLLLRVLGYDQETLVRELSAYGDLVQGLLDEAVLAMRPEEAMVRLFT LLRPGDPPKKDKALAYLFGLLADPKRY
DLGEAGRYKAEEKLGVGLSGRTLVRFEDGEFKDEVFLPTLRYLFALTAGVPGH EVDDIDHLGNRRIRTVGELMADQFRVGLARLARGVR
ERMVMGSPDTLTPAKLVNSRPLEAALREFFSRSQLSQFKDETNP LSSLRHKRRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPE
GANIGLITSLAAYARVDALGFIRTPYRRVKNGVVT EEVVYMTASEEDRYTIAQANTPLEGDRIATDRVVARRRGEPVIVAPEEVEFMDV
SPKQVFSLNTNLIPFLEHDDANRALM GSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDGEVVKVDGTRIAVRYEDGRLVEH
PLRRYARSNQGTAFDQR PRVRVGQRVKKGDLLADGPASEEGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYTSIHIERYEIEA
RDTKLGPE RITRDIPHLSEAALRDLDEEGIVRIGAEVKPGDILVGRTSFKGEQEPSPEERLLRSIFGEKARDVKDTSLRVPPGEGGIV
VGRLRLRRGDPGVELKPGVREVVRVFVAQKRKLQVGDKLANRHGNKGVVAKILPVEDMPHLPDGTPVDVILNPLGVPSRM NLGQILETH
LGLAGYFLGQRYISPVFDGATEPEIKELLAEAFNLYFGKRQGEGFGVDKREKEVLARAEKLGLVSPGKSPE EQLKELFDLGKVVLYDGR
TGEPFEGPIVVGQMFIMKLYHMVEDKMHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWAL EAYGAAHTLQEMLTIKSDDIEGRNAAY
QAIIKGEDVPEPSVPESFRVLVKELQALALDVQTLDEKDNPVDIFEGLASKR
Chain F,L
TSDPVRQYLHEIGQVPLLTLEEEIDLARKVEEGMEAIKKLSEATGLDQELIREVVRAKILGTARIQKIPGLKEKPDPKTV EEVDGKLKS
LPKELKRYLHIAREGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKFEYKRRFKFS TYATWWIRQAINRAIADQ
ARTIRIPVHMVETINKLSRTARQLQQELGREPSYEEIAEAMGPGWDAKRVEETLKIAQEPVS LETPIGDEKDSFYGDFIPDENLPSPVE
AAAQSLLSEELEKALSKLSEREAMVLKLRKGLIDGREHTLEEVGAYFGVTRER IRQIENKALRKLKYHESRTRKLRDFLE
Chain E,K
MAEPGIDKLFGMVDSKYRLTVVVAKRAQQLLRHRFKNTVLEPEERPKMRTLEGLYDDPNAVTWAMKELLTGRLFFGENLV PEDRLQKEM
ERLYPTEEEA
Chain M,N
MKEFRPGDKVVLPPYGVGVVAGIAQRSVSGVSRAYYQVDFPGSRSKAYVPVEAPHSVGLRKALAPEEVPVILDLLKNGRM PLPKQWAAR
HRKTSEILADGNPYRIAQMAGQLRAWEVERGLPDLDRQALRRAIHLLAEEVAQSLEITVQEAKRLFEEAWG EELN
Primary Citation:
Bae, B., Chen, J., Davis, E., Leon, K., Darst, S.A., Campbell, E.A.
CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex. 
Elife, 4, pp. - , 2015.
Experimental Information:
X-RAY DIFFRACTION
Space Group:
P 43 21 2
Cell Constants:
a = 293.15 b = 293.15 c = 539.13 (Ångstroms)
α = 90.0 β = 90.0 γ = 90.0 (degrees)
Refinement:
The structure was refined using the PHENIX program.
The R value is 0.234 for 148127 reflections in
the resolution range 49.79 to 4.01 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)