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NDB ID: 4YIS    PDB ID: 4YIS 


Title:

CRYSTAL STRUCTURE OF LAGLIDADG MEGANUCLEASE I-CPAMI BOUND TO UNCLEAVED DNA

Molecular Description:

Meganuclease I-CpaMI/DNA Complex

Deposited:

2015-03-02

Released:

2016-03-23

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Lambert, A.R., Hallinan, J.P., Shen, B.W., Chik, J.K., Bolduc, J.M., Kulshina, N., Robins, L.I., Kaiser, B.K., Jarjour, J., Havens, K., Scharenberg, A.M., Stoddard, B.L.
Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity. 
Structure, 24, pp. 862 - 873, 2016.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 75.869   b = 91.799   c = 139.636 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.2202 for 20174 reflections in the resolution range 50.0 to 2.89 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)