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NDB ID: 4YLN    PDB ID: 4YLN 


Title:

E. COLI TRANSCRIPTION INITIATION COMPLEX - 17-BP SPACER AND 4-NT RNA

Molecular Description:

E. coli RNA Polymerase alpha subunit
E. coli RNA Polymerase beta subunit
E. coli RNA Polymerase beta' subunit
E. coli RNA Polymerase omega subunit
E. coli RNA Polymerase sigma70 factor/DNA Complex

Deposited:

2015-03-05

Released:

2015-04-22

Structural Keywords:

B DOUBLE HELIX, OPEN HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Zuo, Y., Steitz, T.A.
Crystal Structures of the E. coli Transcription Initiation Complexes with a Complete Bubble. 
Mol.Cell, 58, pp. 534 - 540, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 237.401   b = 206.052   c = 248.69 (Ångstroms)

α = 90.0   β = 116.55   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.24889 for 65966 reflections in the resolution range 39.9 to 5.5 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)