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NDB ID: NA2806    PDB ID: 4NCB 


Title:

STRUCTURE OF THERMUS THERMOPHILUS ARGONAUTE BOUND TO GUIDE DNA AND 19-MER TARGET DNA WITH MG2+

Molecular Description:

Argonaute

Deposited:

2013-10-24

Released:

2014-01-15

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 4 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sheng, G., Zhao, H., Wang, J., Rao, Y., Tian, W., Swarts, D.C., van der Oost, J., Patel, D.J., Wang, Y.
Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage. 
Proc.Natl.Acad.Sci.USA, 111, pp. 652 - 657, 2014.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 84.736   b = 105.363   c = 92.058 (Ångstroms)

α = 90.0   β = 94.25   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1776 for 75196 reflections in the resolution range 39.216 to 2.189 Ångstroms with Fobs >  1.4 sigma(Fobs) and with I >  0.0 sigma(I)