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NDB ID: 4X4S    PDB ID: 4X4S 


Title:

CRYSTAL STRUCTURE OF THE A.FULGIDUS CCA-ADDING ENZYME IN COMPLEX WITH A G70A ARGINYL-TRNA MINIHELIX ENDING IN CCACC AND CTP

Molecular Description:

A.fulgidus CCA-adding enzyme/RNA Complex

Deposited:

2014-12-03

Released:

2015-02-11

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Kuhn, C.D., Wilusz, J.E., Zheng, Y., Beal, P.A., Joshua-Tor, L.
On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme. 
Cell, 160, pp. 644 - 658, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 111.343   b = 216.45   c = 58.447 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2229 for 22667 reflections in the resolution range 48.669 to 3.25 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)