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NDB ID: 5AXW    PDB ID: 5AXW     NAKB: 5AXW 


Title:

CRYSTAL STRUCTURE OF STAPHYLOCOCCUS AUREUS CAS9 IN COMPLEX WITH SGRNA AND TARGET DNA (TTGGGT PAM)

Molecular Description:

CRISPR-associated endonuclease Cas9/RNA/DNA Complex

Deposited:

2015-08-01

Released:

2015-09-02

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Nishimasu, H., Cong, L., Yan, W.X., Ran, F.A., Zetsche, B., Li, Y., Kurabayashi, A., Ishitani, R., Zhang, F., Nureki, O.
Crystal Structure of Staphylococcus aureus Cas9. 
Cell, 162, pp. 1113 - 1126, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 67.596   b = 345.664   c = 98.052 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2046 for 64175 reflections in the resolution range 49.67 to 2.7 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)