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NDB ID: 5CCX    PDB ID: 5CCX 


Title:

STRUCTURE OF THE PRODUCT COMPLEX OF TRNA M1A58 METHYLTRANSFERASE WITH TRNA3LYS AS SUBSTRATE

Molecular Description:

tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A
tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6/RNA Complex

Deposited:

2015-07-02

Released:

2015-11-04

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Finer-Moore, J., Czudnochowski, N., O'Connell, J.D., Wang, A.L., Stroud, R.M.
Crystal Structure of the Human tRNA m(1)A58 Methyltransferase-tRNA3(Lys) Complex: Refolding of Substrate tRNA Allows Access to the Methylation Target. 
J.Mol.Biol., 427, pp. 3862 - 3876, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 43 21 2

Cell Constants:

a = 136.77   b = 136.77   c = 177.16 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.208 for 98079 reflections in the resolution range 54.13 to 2.1 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)