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NDB ID: 5CD4    PDB ID: 5CD4 


Title:

THE TYPE IE CRISPR CASCADE COMPLEX FROM E. COLI, WITH TWO ASSEMBLIES IN THE ASYMMETRIC UNIT ARRANGED BACK-TO-BACK

Molecular Description:

CRISPR system Cascade subunit CasE
CRISPR system Cascade subunit CasC
CRISPR system Cascade subunit CasD
CRISPR system Cascade subunit CasA
CRISPR system Cascade subunit CasB/RNA Complex

Deposited:

2015-07-03

Released:

2015-08-26

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

van Erp, P.B., Jackson, R.N., Carter, J., Golden, S.M., Bailey, S., Wiedenheft, B.
Mechanism of CRISPR-RNA guided recognition of DNA targets in Escherichia coli. 
Nucleic Acids Res., 43, pp. 8381 - 8391, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 105.99   b = 244.8   c = 426.74 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2127 for 183492 reflections in the resolution range 49.735 to 3.2 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)