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NDB ID: 5DH7    PDB ID: 5DH7     NAKB(beta): 5DH7 


Title:

TWO DIVALENT METAL IONS AND CONFORMATIONAL CHANGES PLAY ROLES IN THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION-G12A MUTANT IN MN2+

Molecular Description:

RNA

Deposited:

2015-08-29

Released:

2015-10-07

Structural Keywords:

A DOUBLE HELIX, BULGE

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

No Protein Sequence Found

Primary Citation:

Mir, A., Chen, J., Robinson, K., Lendy, E., Goodman, J., Neau, D., Golden, B.L.
Two Divalent Metal Ions and Conformational Changes Play Roles in the Hammerhead Ribozyme Cleavage Reaction. 
Biochemistry, 54, pp. 6369 - 6381, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 81.309   b = 85.825   c = 103.528 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2053 for 12124 reflections in the resolution range 40.655 to 3.064 Ångstroms with Fobs >  1.39 sigma(Fobs) and with I >  0.0 sigma(I)