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NDB ID: 5DS4    PDB ID: 5DS4 


Title:

CRYSTAL STRUCTURE THE ESCHERICHIA COLI CAS1-CAS2 COMPLEX BOUND TO PROTOSPACER DNA

Molecular Description:

CRISPR-associated endonuclease Cas1
CRISPR-associated endoribonuclease Cas2/DNA Complex

Deposited:

2015-09-16

Released:

2015-10-28

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Nunez, J.K., Harrington, L.B., Kranzusch, P.J., Engelman, A.N., Doudna, J.A.
Foreign DNA capture during CRISPR-Cas adaptive immunity. 
Nature, 527, pp. 535 - 538, 2015.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 88.02   b = 123.006   c = 196.012 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2438 for 35770 reflections in the resolution range 49.003 to 3.2 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)