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NDB ID: 5FW2    PDB ID: 5FW2 


Title:

CRYSTAL STRUCTURE OF SPCAS9 VARIANT EQR BOUND TO SGRNA AND TGAG PAM TARGET DNA

Molecular Description:

CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1/RNA Complex

Deposited:

2016-02-11

Released:

2016-06-15

Structural Keywords:

DOUBLE HELIX, HAIRPIN LOOP, INTERNAL LOOP

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Anders, C., Bargsten, K., Jinek, M.
Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9. 
Mol.Cell, 61, pp. 895 - , 2016.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 177.74   b = 68.24   c = 188.22 (Ångstroms)

α = 90.0   β = 110.95   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2064 for 59668 reflections in the resolution range 48.251 to 2.676 Ångstroms with Fobs >  1.11 sigma(Fobs) and with I >  0.0 sigma(I)