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NDB ID: 5IPN    PDB ID: 5IPN 


Title:

SIGMAS-TRANSCRIPTION INITIATION COMPLEX WITH 4-NT NASCENT RNA

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor RpoS/DNA Complex

Deposited:

2016-03-09

Released:

2016-03-30

Structural Keywords:

DOUBLE HELIX, OPEN HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Liu, B., Zuo, Y., Steitz, T.A.
Structures of E. coli sigma S-transcription initiation complexes provide new insights into polymerase mechanism. 
Proc.Natl.Acad.Sci.USA, 113, pp. 4051 - 4056, 2016.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 132.874   b = 152.17   c = 229.12 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.28316 for 24772 reflections in the resolution range 126.76 to 4.61 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)