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NDB ID: 5O7T    PDB ID: 5O7T 


Title:

CRYSTAL STRUCTURE OF KLENTAQ MUTANT M747K IN A CLOSED TERNARY COMPLEX WITH A DG:DCTP BASE PAIR

Molecular Description:

DNA polymerase I
thermostable/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Betz, K., Nilforoushan, A., Wyss, L.A., Diederichs, K., Sturla, S.J., Marx, A.
Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase. 
Chem. Commun. (Camb.), 53, pp. 12704 - 12707, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 31 2 1

Cell Constants:

a = 109.476   b = 109.476   c = 91.39 (Ångstroms)

α = 90.0   β = 90.0   γ = 120.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1799 for 112015 reflections in the resolution range 47.404 to 1.8 Ångstroms with Fobs >  1.26 sigma(Fobs) and with I >  0.0 sigma(I)