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NDB ID: 5OR5    PDB ID: 5OR5 


Title:

NMR STRUCTURE OF THE COMPLEX FORMED BY AN ENGINEERED REGION 2 OF SIGMAE IN COMPLEX WITH GTAAAA

Molecular Description:

ECF RNA polymerase sigma-E factor
ECF RNA polymerase sigma factor SigW
ECF RNA polymerase sigma-E factor/DNA Complex

Structural Keywords:

SMALL NA FRAGMENT

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Campagne, S., Vorholt, J.A., Allain, F.H.
Engineered promoter selectivity of an ECF sigma factor
To be published, , pp. - , 0.

Experimental Information:

SOLUTION NMR

Cell Constants:

a =   b =   c = (Ångstroms)

α =   β =   γ = (degrees)

Number of Models:

Number of Models: 13 Structures

Sample Details:

1 mM [U-99% 13C; U-99% 15N] Engineered region2 of sigmaE from E. coli in which the loop L3 was replaced by the loop L3 of Bacillus subtilis sigmaW, 1 mM DNA (5'-D(*GP*TP*AP*AP*AP*A)-3'), 10 mM sodium phosphate, 50 mM sodium chloride, 90% H2O/10% D2O 90% H2O/10% D2O