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NDB ID: 5VAJ    PDB ID: 5VAJ 


Title:

BHRNASE H - AMIDE-RNA/DNA COMPLEX

Molecular Description:

Ribonuclease H/RNA Complex

Structural Keywords:

A DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Mutisya, D., Hardcastle, T., Cheruiyot, S.K., Pallan, P.S., Kennedy, S.D., Egli, M., Kelley, M.L., Smith, A.V.B., Rozners, E.
Amide linkages mimic phosphates in RNA interactions with proteins and are well tolerated in the guide strand of short interfering RNAs. 
Nucleic Acids Res., 45, pp. 8142 - 8155, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 137.18   b = 37.513   c = 93.863 (Ångstroms)

α = 90.0   β = 121.65   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.16813 for 28679 reflections in the resolution range 79.9 to 1.95 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)