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NDB ID: 5VI5    PDB ID: 5VI5 

Title:

STRUCTURE OF MYCOBACTERIUM SMEGMATIS TRANSCRIPTION INITIATION COMPLEX WITH A FULL TRANSCRIPTION BUBBLE

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor SigA
RNA polymerase-binding protein RbpA/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 6 protein sequences

Primary Citation:

Hubin, E.A., Lilic, M., Darst, S.A., Campbell, E.A.
Structural insights into the mycobacteria transcription initiation complex from analysis of X-ray crystal structures. 
Nat Commun, 8, pp. 16072 - 16072, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 132.062   b = 163.555   c = 139.964 (Ångstroms)

α = 90.0   β = 107.9   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.254 for 87525 reflections in the resolution range 50.015 to 3.196 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)

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