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NDB ID: 5VO8    PDB ID: 5VO8 


Title:

X-RAY CRYSTAL STRUCTURE OF A BACTERIAL REITERATIVE TRANSCRIPTION COMPLEX OF PYRG PROMOTER

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor SigA/DNA Complex

Structural Keywords:

DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Murakami, K.S., Shin, Y., Turnbough, C.L., Molodtsov, V.
X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway. 
Proc. Natl. Acad. Sci. U.S.A., 114, pp. 8211 - 8216, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 186.537   b = 101.984   c = 296.113 (Ångstroms)

α = 90.0   β = 98.56   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1865 for 78219 reflections in the resolution range 29.883 to 3.3 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)