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NDB ID: 5VVJ    PDB ID: 5VVJ 


Title:

CAS1-CAS2 BOUND TO HALF-SITE INTERMEDIATE

Molecular Description:

CRISPR-associated endonuclease Cas1
CRISPR-associated endoribonuclease Cas2/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Wright, A.V., Liu, J.J., Knott, G.J., Doxzen, K.W., Nogales, E., Doudna, J.A.
Structures of the CRISPR genome integration complex. 
Science, 357, pp. 1113 - 1118, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 75.067   b = 183.113   c = 196.92 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2903 for 25669 reflections in the resolution range 134.096 to 3.89 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)