## NDB ID: 5WCU PDB ID: 5WCU

### Title:

CRYSTAL STRUCTURE OF 167 BP NUCLEOSOME BOUND TO THE GLOBULAR DOMAIN OF LINKER HISTONE H5

### Molecular Description:

Histone H3

Histone H4

Histone H2A

Histone H2B

Histone H5/DNA Complex

### Deposited:

2017-07-02

### Released:

2018-10-31

### Structural Keywords:

B DOUBLE HELIX

### Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Chain I,S

(DA)(DT)(DC)(DG)(DG)(DC)(DC)(DG)(DC)(DC)(DA)(DT)(DC)(DG)(DA)(DG)(DA)(DA)(DT)(DC) (DC)(DC)(

DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA) (DT)(DT)(DG)(DG)(D

T)(DC)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG) (DC)(DA)(DC)(DC)(DG)(DC)(DT

)(DT)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC) (DG)(DC)(DG)(DC)(DT)(DG)(DT)(DC)(DC)

(DC)(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA) (DA)(DC)(DC)(DG)(DC)(DC)(DA)(DA)(DG)(DG)(DG)(

DG)(DA)(DT)(DT)(DA)(DC)(DT)(DC)(DC) (DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG)(DG)(DC)(D

A)(DC)(DG)(DT)(DG)(DT)(DC) (DA)(DG)(DA)(DT)(DA)(DT)(DA)(DT)(DA)(DC)(DA)(DT)(DC)(DC)(DG)(DA

)(DT)(DG)(DC)(DA) (DT)(DG)(DT)(DA)(DG)(DA)(DT)

Chain J,T

(DA)(DT)(DC)(DT)(DA)(DC)(DA)(DT)(DG)(DC)(DA)(DT)(DC)(DG)(DG)(DA)(DT)(DG)(DT)(DA) (DT)(DA)(

DT)(DA)(DT)(DC)(DT)(DG)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG) (DA)(DG)(DA)(DC)(D

T)(DA)(DG)(DG)(DG)(DA)(DG)(DT)(DA)(DA)(DT)(DC)(DC)(DC)(DC)(DT) (DT)(DG)(DG)(DC)(DG)(DG)(DT

)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DG)(DG)(DG) (DA)(DC)(DA)(DG)(DC)(DG)(DC)(DG)(DT)

(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA) (DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(DA)(DG)(

DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA) (DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)(DG)(DC)(D

G)(DG)(DC)(DC)(DT)(DC)(DG) (DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DT)(DC)(DT)(DC)(DG)(DA

)(DT)(DG)(DG)(DC) (DG)(DG)(DC)(DC)(DG)(DA)(DT)

### Protein Sequence:

Click to show/hide 5 protein sequences

Chain A,E,K,O

PHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRV TIMPKDIQL

ARRIRGERA

Chain C,G,M,Q

KSRSNRAGLQFPVGRIHRLLRKGNYAERVGAGAPVYLAAVMEYLAAEVLELAGNAARDNKKTRIIPRHLQLAIRNDEELN KLLSGVTIA

QGGVLPNIQAVLLPK

Chain B,F,L,P

VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGF GG

Chain D,H,N,R

RKRKESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHA VSEGTKAVT

KYTSS

Chain U,V

SASHPTYSEMIAAAIRAEKSRGGSSRQSIQKYIKSHYKVGHNADLQIKLSIRRLLAAGVLKQTKGVGASGSFRLAK

### Primary Citation:

Zhou, B.R., Jiang, J., Ghirlando, R., Norouzi, D., Sathish Yadav, K.N., Feng, H., Wang, R., Zhang, P., Zhurkin, V., Bai, Y.

Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures.

*J. Mol. Biol.*, **430**, pp. 3093 - 3110, 2018.

### Experimental Information:

X-RAY DIFFRACTION

### Space Group:

P 1

### Cell Constants:

a = 65.926 b = 108.543 c = 180.77 (Ångstroms)

α = 100.79 β = 90.08 γ = 89.94 (degrees)

### Refinement:

The structure was refined using the PHENIX program.
The R value is 0.195 for 15266 reflections in
the resolution range 48.62 to 5.53 Ångstroms
with Fobs > 1.93 sigma(Fobs) and with I > 0.0 sigma(I)