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NDB ID: 5XH6    PDB ID: 5XH6 


Title:

CRYSTAL STRUCTURE OF THE ACIDAMINOCOCCUS SP. BV3L6 CPF1 RVR VARIANT IN COMPLEX WITH CRRNA AND TARGET DNA (TATA PAM)

Molecular Description:

CRISPR-associated endonuclease Cpf1/RNA/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Nishimasu, H., Yamano, T., Gao, L., Zhang, F., Ishitani, R., Nureki, O.
Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1 
Mol. Cell, 67, pp. 139 - 147.e2, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 81.162   b = 133.676   c = 199.633 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1767 for 143851 reflections in the resolution range 39.99 to 2.0 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)