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NDB ID: 5XOW    PDB ID: 5XOW 


Title:

CRYSTAL STRUCTURE OF T. THERMOPHILUS ARGONAUTE PROTEIN COMPLEXED WITH A BULGE 6'A7' ON THE TARGET STRAND

Molecular Description:

TtAgo (D546N)/DNA Complex

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sheng, G., Gogakos, T., Wang, J., Zhao, H., Serganov, A., Juranek, S., Tuschl, T., Patel, D.J., Wang, Y.
Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes 
Nucleic Acids Res., 45, pp. 9149 - 9163, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 41 3 2

Cell Constants:

a = 200.824   b = 200.824   c = 200.824 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2164 for 30962 reflections in the resolution range 48.707 to 2.902 Ångstroms with Fobs >  1.47 sigma(Fobs) and with I >  0.0 sigma(I)