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NDB ID: 5XVP    PDB ID: 5XVP 


Title:

E. FAE CAS1-CAS2/PRESPACER/TARGET TERNARY COMPLEX REVEALING THE FULLY INTEGRATED STATES

Molecular Description:

CRISPR-associated endonuclease Cas1
CRISPR-associated endoribonuclease Cas2/DNA Complex

Structural Keywords:

B DOUBLE HELIX, OVERHANGING BASES

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Xiao, Y., Ng, S., Hyun Nam, K., Ke, A.
How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration. 
Nature, 550, pp. 137 - 141, 2017.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

I 2 2 2

Cell Constants:

a = 64.87   b = 212.294   c = 512.199 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.19557 for 68098 reflections in the resolution range 49.03 to 3.0 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)