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NDB ID: 5E63    PDB ID: 5E63 


Title:

K262A MUTANT OF I-SMAMI

Molecular Description:

I-SmaMI LAGLIDADG meganuclease/DNA Complex

Deposited:

2015-10-09

Released:

2016-01-13

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 4 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Shen, B.W., Lambert, A., Walker, B.C., Stoddard, B.L., Kaiser, B.K.
The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease. 
J.Mol.Biol., 428, pp. 206 - 220, 2016.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 59.835   b = 67.694   c = 97.78 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.19296 for 12093 reflections in the resolution range 30.0 to 2.6 Ångstroms with Fobs >  1.0 sigma(Fobs) and with I >  0.0 sigma(I)