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NDB ID: 6AI6    PDB ID: 6AI6 


Title:

CRYSTAL STRUCTURE OF SPCAS9-NG

Molecular Description:

CRISPR-associated endonuclease Cpf1/RNA/DNA Complex

Structural Keywords:

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Nishimasu, H., Shi, X., Ishiguro, S., Gao, L., Hirano, S., Okazaki, S., Noda, T., Abudayyeh, O.O., Gootenberg, J.S., Mori, H., Oura, S., Holmes, B., Tanaka, M., Seki, M., Hirano, H., Aburatani, H., Ishitani, R., Ikawa, M., Yachie, N., Zhang, F., Nureki, O.
Engineered CRISPR-Cas9 nuclease with expanded targeting space 
Science, 361, pp. 1259 - 1262, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 177.377   b = 68.568   c = 187.968 (Ångstroms)

α = 90.0   β = 111.51   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2193 for 58087 reflections in the resolution range 48.166 to 2.7 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)