NDB ID: 6CAR PDB ID: 6CAR 
NAKB(beta): 6CAR 
Title:
SERIAL FEMTOSECOND X-RAY CRYSTAL STRUCTURE OF 30S RIBOSOMAL SUBUNIT FROM THERMUS THERMOPHILUS IN COMPLEX WITH SISOMICIN
Molecular Description:
30S ribosomal protein S2
30S ribosomal protein S3
30S ribosomal protein S4
30S ribosomal protein S5
30S ribosomal protein S6
30S ribosomal protein S7
30S ribosomal protein S8
30S ribosomal protein S9
30S ribosomal protein S10
30S ribosomal protein S11
30S ribosomal protein S12
30S ribosomal protein S13
30S ribosomal protein S14 type Z
30S ribosomal protein S15
30S ribosomal protein S16
30S ribosomal protein S17
30S ribosomal protein S18
30S ribosomal protein S19
30S ribosomal protein S20
30S ribosomal protein Thx/RNA Complex
Deposited:
2018-01-31
Released:
2018-07-25
Nucleic Acid Sequence:
Click to show/hide 3 nucleic acid sequences
Chain Y
UUUUUU
Chain W
GGGAUUGAAAAUCCC
Chain A
UGGAGAGUCUGAUCCUGGCUCAGGGUGAACGCUGGCGGCGUGCCUAAGACAUGCAAGUCGUGCGGGCCGCGGGGUUUUAC UCCGUGGUC
AGCGGCGGACGGGUGAGUAACGCGUGGGUGACCUACCCGGAAGAGGGGGACAACCCGGGGAAACUCGGGCU AAUCCCCCAUGUGGACCC
GCCCCUUGGGGUGUGUCCAAAGGGCUUUGCCCGCUUCCGGAUGGGCCCGCGUCCCAUCAGCU AGUUGGUGGGGUAAUGGCCCACCAAGG
CGACGACGGGUAGCCGGUCUGAGAGGAUGGCCGGCCACAGGGGCACUGAGACA CGGGCCCCACUCCUACGGGAGGCAGCAGUUAGGAAU
CUUCCGCAAUGGGCGCAAGCCUGACGGAGCGACGCCGCUUGGAG GAAGAAGCCCUUCGGGGUGUAAACUCCUGAACCCGGGACGAAACC
CCCGACGAGGGGACUGACGGUACCGGGGUAAUAGC GCCGGCCAACUCCG(PSU)GCCAGCAGCC(G7M)CGGUAAUACGGAGGGCGCGA
GCGUUACCCGGAUUCACUGGGCGUAA AGGGCGUGUAGGCGGCCUGGGGCGUCCCAUGUGAAAGACCACGGCUCAACCGUGGGGGAGCGU
GGGAUACGCUCAGGCUA GACGGUGGGAGAGGGUGGUGGAAUUCCCGGAGUAGCGGUGAAAUGCGCAGAUACCGGGAGGAACGCCGAUGG
CGAAGGCA GCCACCUGGUCCACCCGUGACGCUGAGGCGCGAAAGCGUGGGGAGCAAACCGGAUUAGAUACCCGGGUAGUCCACGCCCU
AAACGAUGCGCGCUAGGUCUCUGGGUCUCCUGGGGGCCGAAGCUAACGCGUUAAGCGCGCCGCCUGGGGAGUACGGCCGC AAGGCUGAA
ACUCAAAGGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUUCGAA(M2G)(5MC)AACG CGAAGAACCUUACCAGGC
CUUGACAUGCUAGGGAACCCGGGUGAAAGCCUGGGGUGCCCCGCGAGGGGAGCCCUAGCACA GGUGCUGCAUGGCCGUCGUCAGCUCGU
GCCGUGAGGUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCCCGCCGUUAGUU GCCAGCGGUUCGGCCGGGCACUCUAACGGGACUGCC
CGCGAAAGCGGGAGGAAGGAGGGGACGACGUCUGGUCAGCAUG (2MG)CCCUUACGGCCUGGGCGACACACGUGCUACAAUGCCCACUA
CAAAGCGAUGCCACCCGGCAACGGGGAGCUAAUC GCAAAAAGGUGGGCCCAGUUCGGAUUGGGGUCUGCAACCCGACCCCAUGAAGCCG
GAAUCGCUAGUAAUCGCGGAUCAGC CAUGCCGCGGUGAAUACGUUCCCGGGCCUUGUACACAC(5MC)G(4OC)C(5MC)GU(5MC)AC
GCCAUGGGAGCGGGCU CUACCCGAAGUCGCCGGGAGCCUACGGGCAGGCGCCGAGGGUAGGGCCCGUGACUGGGGCGAAGUCG(UR3)A
ACAAGGU AGCUGUACCGG(MA6)(MA6)GGUGCGGCUGGAUCACCUCC(PSU)(PSU)UCU
Protein Sequence:
Click to show/hide 20 protein sequences
Chain T
AQKKPKRNLSALKRHRQSLKRRLRNKAKKSAIKTLSKKAIQLAQEGKAEEALKIMRKAESLIDKAAKGSTLHKNAAARRK SRLMRKVRQ
LLEAAGAPLIGGGLSA
Chain U
GKGDRRTRRGKIWRGTYGKYRPRKKK
Chain C
GNKIHPIGFRLGITRDWESRWYAGKKQYRHLLLEDQRIRGLLEKELYSAGLARVDIERAADNVAVTVHVAKPGVVIGRGG ERIRVLREE
LAKLTGKNVALNVQEVQNPNLSAPLVAQRVAEQIERRFAVRRAIKQAVQRVMESGAKGAKVIVSGRIGGAE QARTEWAAQGRVPLHTLR
ANIDYGFALARTTYGVLGVKAYIFLGEVIGGQKPKARPELPKAEERPRRRRPAVRVKKEE
Chain B
PVEITVKELLEAGVHFGHERKRWNPKFARYIYAERNGIHIIDLQKTMEELERTFRFIEDLAMRGGTILFVGTKKQAQDIV RMEAERAGM
PYVNQRWLGGMLTNFKTISQRVHRLEELEALFASPEIEERPKKEQVRLKHELERLQKYLSGFRLLKRLPDA IFVVDPTKEAIAVREARK
LFIPVIALADTDSDPDLVDYIIPGNDDAIRSIQLILSRAVDLIIQARGGVVEPSPSYALVQE AEATETPEGESEVEA
Chain E
PETDFEEKMILIRRTARMQAGGRRFRFGALVVVGDRQGRVGLGFGKAPEVPLAVQKAGYYARRNMVEVPLQNGTIPHEIE VEFGASKIV
LKPAAPGTGVIAGAVPRAILELAGVTDILTKELGSRNPINIAYATMEALRQLRTKADVERLRKGEAHAQAQ G
Chain D
GRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRPSDYAVRLREKQKLRRIYGISERQFRNLFEE ASKKKGVTG
SVFLGLLESRLDNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEKSRNLELIRQN LEAMKGRKVGPWLSLDVE
GMKGKFLRLPDREDLALPVNEQLVIEFYSR
Chain G
ARRRRAEVRQLQPDLVYGDVLVTAFINKIMRDGKKNLAARIFYDACKIIQEKTGQEPLKVFKQAVENVKPRMEVRSRRVG GANYQVPME
VSPRRQQSLALRWLVQAANQRPERRAAVRIAHELMDAAEGKGGAVKKKEDVERMAEANRAYAHYRW
Chain F
MRRYEVNIVLNPNLDQSQLALEKEIIQRALENYGARVEKVEELGLRRLAYPIAKDPQGYFLWYQVEMPEDRVNDLARELR IRDNVRRVM
VVKSQEPFLANA
Chain I
EQYYGTGRRKEAVARVFLRPGNGKVTVNGQDFNEYFQGLVRAVAALEPLRAVDALGHFDAYITVRGGGKSGQIDAIKLGI ARALVQYNP
DYRAKLKPLGFLTRDARVVERKKYGKHKARRAPQYSKR
Chain H
MLTDPIADMLTRIRNATRVYKESTDVPASRFKEEILRILAREGFIKGYERVDVDGKPYLRVYLKYGPRRQGPDPRPEQVI HHIRRISKP
GRRVYVGVKEIPRVRRGLGIAILSTSKGVLTDREARKLGVGGELICEVW
Chain K
AKKPSKKKVKRQVASGRAYIHASYNNTIVTITDPDGNPITWSSGGVIGYKGSRKGTPYAAQLAALDAAKKAMAYGMQSVD VIVRGTGAG
REQAIRALQASGLQVKSIVDDTPVPHNGCRPKKKFRKAS
Chain J
PKIRIKLRGFDHKTLDASAQKIVEAARRSGAQVSGPIPLPTRVRRFTVIRGPFKHKDSREHFELRTHNRLVDIINPNRKT IEQLMTLDL
PTGVEIEIKTVGGGR
Chain M
ARIAGVEIPRNKRVDVALTYIYGIGKARAKEALEKTGINPATRVKDLTEAEVVRLREYVENTWKLEGELRAEVAANIKRL MDIGCYRGL
RHRRGLPVRGQRTRTNARTRKGPRKTVAGKKKAPRK
Chain L
PTINQLVRKGREKVRKKSKVPALKGAPFRRGVCTVVRTVTPKKPNSALRKVAKVRLTSGYEVTAYIPGEGHNLQEHSVVL IRGGRVK(0
TD)LPGVRYHIVRGVYDAAGVKDRKKSRSKYGTKKPKEAAKTAAKK
Chain O
PITKEEKQKVIQEFARFPGDTGSTEVQVALLTLRINRLSEHLKVHKKDHHSHRGLLMMVGQRRRLLRYLQREDPERYRAL IEKLGIRG
Chain N
ARKALIEKAKRTPKFKVRAYTRCVRCGRARSVYRFFGLCRICLRELAHKGQLPGVRKASW
Chain Q
PKKVLTGVVVSDKMQKTVTVLVERQFPHPLYGKVIKRSKKYLAHDPEEKYKLGDVVEIIESRPISKRKRFRVLRLVESGR MDLVEKYLI
RRQNYQSLSKRGGKA
Chain P
MVKIRLARFGSKHNPHYRIVVTDARRKRDGKYIEKIGYYDPRKTTPDWLKVDVERARYWLSVGAQPTDTARRLLRQAGVF RQEAREGA
Chain S
PRSLKKGVFVDDHLLEKVLELNAKGEKRLIKTWSRRSTIVPEMVGHTIAVYNGKQHVPVYITENMVGHKLGEFAPTRTYR GHGKEAKAT
KKK
Chain R
STKNAKPKKEAQRRPSRKAKVKATLGEFDLRDYRNVEVLKRFLSETGKILPRRRTGLSAKEQRILAKTIKRARILGLLPF TEKLVRK
Primary Citation:
O'Sullivan, M.E., Poitevin, F., Sierra, R.G., Gati, C., Dao, E.H., Rao, Y., Aksit, F., Ciftci, H., Corsepius, N., Greenhouse, R., Hayes, B., Hunter, M.S., Liang, M., McGurk, A., Mbgam, P., Obrinsky, T., Pardo-Avila, F., Seaberg, M.H., Cheng, A.G., Ricci, A.J., DeMirci, H.
Aminoglycoside ribosome interactions reveal novel conformational states at ambient temperature. 
Nucleic Acids Res., 46, pp. 9793 - 9804, 2018.
Experimental Information:
X-RAY DIFFRACTION
Space Group:
P 41 21 2
Cell Constants:
a = 402.6 b = 402.6 c = 175.9 (Ångstroms)
α = 90.0 β = 90.0 γ = 90.0 (degrees)
Refinement:
The structure was refined using the PHENIX program.
The R value is 0.2219 for 196314 reflections in
the resolution range 39.262 to 3.4 Ångstroms
with Fobs > 1.33 sigma(Fobs) and with I > 0.0 sigma(I)