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NDB ID: 6DCF    PDB ID: 6DCF 


Title:

CRYSTAL STRUCTURE OF A MYCOBACTERIUM SMEGMATIS TRANSCRIPTION INITIATION COMPLEX WITH RIFAMPICIN-RESISTANT RNA POLYMERASE AND BOUND TO KANGLEMYCIN A

Molecular Description:

RNA polymerase-binding protein RbpA
DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor SigA/DNA Complex

Deposited:

2018-05-06

Released:

2018-09-05

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 6 protein sequences

Primary Citation:

Peek, J., Lilic, M., Montiel, D., Milshteyn, A., Woodworth, I., Biggins, J.B., Ternei, M.A., Calle, P.Y., Danziger, M., Warrier, T., Saito, K., Braffman, N., Fay, A., Glickman, M.S., Darst, S.A., Campbell, E.A., Brady, S.F.
Rifamycin congeners kanglemycins are active against rifampicin-resistant bacteria via a distinct mechanism. 
Nat Commun, 9, pp. 4147 - 4147, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 129.25   b = 160.909   c = 136.864 (Ångstroms)

α = 90.0   β = 110.75   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.252 for 67452 reflections in the resolution range 43.89 to 3.45 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)