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NDB ID: 6EKO    PDB ID: 6EKO 


Title:

CRYSTAL STRUCTURE OF TYPE IIP RESTRICTION ENDONUCLEASE PFOI WITH COGNATE DNA

Molecular Description:

restriction endonuclease PfoI (E.C.3.1.21.4)

Deposited:

2017-09-26

Released:

2018-10-10

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 1 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Tamulaitiene, G., Manakova, E., Jovaisaite, V., Tamulaitis, G., Grazulis, S., Bochtler, M., Siksnys, V.
Unique mechanism of target recognition by PfoI restriction endonuclease of the CCGG-family. 
Nucleic Acids Res., 47, pp. 997 - 1010, 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 56.279   b = 91.663   c = 152.742 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2094 for 36082 reflections in the resolution range 47.96 to 2.284 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)