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NDB ID: 6EMZ    PDB ID: 6EMZ 


Title:

STRUCTURE OF THE TN1549 TRANSPOSON INTEGRASE (AA 82-397, R225K) IN COMPLEX WITH CIRCULAR INTERMEDIATE DNA (CI5-DNA)

Molecular Description:

Int protein/DNA Complex

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Rubio-Cosials, A., Schulz, E.C., Lambertsen, L., Smyshlyaev, G., Rojas-Cordova, C., Forslund, K., Karaca, E., Bebel, A., Bork, P., Barabas, O.
Transposase-DNA Complex Structures Reveal Mechanisms for Conjugative Transposition of Antibiotic Resistance. 
Cell, 173, pp. 208 - 220.e20, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 64.59   b = 125.06   c = 77.98 (Ångstroms)

α = 90.0   β = 96.53   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1994 for 29672 reflections in the resolution range 48.357 to 2.79 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)