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NDB ID: 6FB0    PDB ID: 6FB0 


Title:

CRYSTAL STRUCTURE OF A TAILORED I-CREI HOMING ENDONUCLEASE PROTEIN (3115 VARIANT) IN COMPLEX WITH ITS TARGET DNA (HAEMOGLOBIN BETA SUBUNIT GENE) IN THE PRESENCE OF CALCIUM

Molecular Description:

Penicillin-binding protein 2

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Prieto, J., Redondo, P., Lopez-Mendez, B., D'Abramo, M., Merino, N., Blanco, F.J., Duchateau, P., Montoya, G., Molina, R.
Understanding the indirect DNA read-out specificity of I-CreI Meganuclease. 
Sci Rep, 8, pp. 10286 - 10286, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 45.437   b = 67.673   c = 83.525 (Ångstroms)

α = 90.0   β = 96.75   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1799 for 26697 reflections in the resolution range 33.837 to 2.15 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)