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NDB ID: 6FB6    PDB ID: 6FB6 


Title:

CRYSTAL STRUCTURE OF A TAILORED I-CREI HOMING ENDONUCLEASE PROTEIN (3115 VARIANT) IN COMPLEX WITH AN ALTERED VERSION OF ITS TARGET DNA (HAEMOGLOBIN BETA SUBUNIT GENE) AT 5NNN REGION IN THE PRESENCE OF MANGANESE

Molecular Description:

Penicillin-binding protein 2

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 4 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Prieto, J., Redondo, P., Lopez-Mendez, B., D'Abramo, M., Merino, N., Blanco, F.J., Duchateau, P., Montoya, G., Molina, R.
Understanding the indirect DNA read-out specificity of I-CreI Meganuclease. 
Sci Rep, 8, pp. 10286 - 10286, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 45.176   b = 67.987   c = 83.044 (Ångstroms)

α = 90.0   β = 96.45   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1978 for 28700 reflections in the resolution range 44.89 to 2.6 Ångstroms with Fobs >  1.36 sigma(Fobs) and with I >  0.0 sigma(I)