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NDB ID: 6FB7    PDB ID: 6FB7 


Title:

CRYSTAL STRUCTURE OF THE I-CREI HOMING ENDONUCLEASE D75N VARIANT IN COMPLEX WITH ITS TARGET DNA IN THE PRESENCE OF MANGANESE

Molecular Description:

Penicillin-binding protein 2

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Prieto, J., Redondo, P., Lopez-Mendez, B., D'Abramo, M., Merino, N., Blanco, F.J., Duchateau, P., Montoya, G., Molina, R.
Understanding the indirect DNA read-out specificity of I-CreI Meganuclease. 
Sci Rep, 8, pp. 10286 - 10286, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 71.504   b = 169.153   c = 49.015 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1677 for 30910 reflections in the resolution range 49.015 to 2.689 Ångstroms with Fobs >  0.86 sigma(Fobs) and with I >  0.0 sigma(I)