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NDB ID: 6FI8    PDB ID: 6FI8 


Title:

CRYSTAL STRUCTURE OF THE IS608 TRANSPOSASE IN COMPLEX WITH LEFT END 29-MER DNA HAIRPIN AND A 6-MER DNA REPRESENTING THE INTACT TARGET SITE: PRE-CLEAVAGE TARGET CAPTURE COMPLEX

Molecular Description:

Putative transposase/DNA Complex

Structural Keywords:

RH DOUBLE HELIX, HAIRPIN LOOP

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Morero, N.R., Zuliani, C., Kumar, B., Bebel, A., Okamoto, S., Guynet, C., Hickman, A.B., Chandler, M., Dyda, F., Barabas, O.
Targeting IS608 transposon integration to highly specific sequences by structure-based transposon engineering. 
Nucleic Acids Res., 46, pp. 4152 - 4163, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 41 21 2

Cell Constants:

a = 138.64   b = 138.64   c = 117.95 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.199 for 35890 reflections in the resolution range 89.837 to 2.598 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)