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NDB ID: 6GCF    PDB ID: 6GCF 


Title:

DNA BINDING DOMAIN OF RESTRICTION ENDONUCLEASE MCRBC IN COMPLEX WITH N4-METHYLCYTOSINE DNA

Molecular Description:

5-methylcytosine-specific restriction enzyme B/DNA Complex

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Zagorskaite, E., Manakova, E., Sasnauskas, G.
Recognition of modified cytosine variants by the DNA-binding domain of methyl-directed endonuclease McrBC. 
FEBS Lett., 592, pp. 3335 - 3345, 2018.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 36.28   b = 69.675   c = 145.054 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1389 for 108317 reflections in the resolution range 62.81 to 1.55 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)