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NDB ID: 6I1K    PDB ID: 6I1K 


Title:

CRYSTAL STRUCTURE OF CATALYTICALLY INACTIVE FNCAS12A IN COMPLEX WITH A CRRNA GUIDE AND A DSDNA TARGET

Molecular Description:

CRISPR-associated endonuclease Cas12a/RNA Complex

Deposited:

2018-10-29

Released:

2019-01-23

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Swarts, D.C., Jinek, M.
Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a. 
Mol. Cell, 73, pp. 589 - 600.e4, 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 82.172   b = 141.681   c = 89.478 (Ångstroms)

α = 90.0   β = 97.43   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.249 for 58699 reflections in the resolution range 47.612 to 2.65 Ångstroms with Fobs >  1.34 sigma(Fobs) and with I >  0.0 sigma(I)