NDB ID: 6IDO    PDB ID: 6IDO     NAKB: 6IDO 

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Title:

CRYSTAL STRUCTURE OF KLEBSIELLA PNEUMONIAE SIGMA4 OF SIGMAS FUSING WITH THE RNA POLYMERASE BETA-FLAP-TIP-HELIX IN COMPLEX WITH -35 ELEMENT DNA

Molecular Description:

RNA polymerase sigma factor RpoS
RNA polymerase beta-flap-tip-helix
RNA polymerase sigma factor RpoS
sigma4 of sigmaS fusing with the RNA polymerase beta-flap-tip-helix/DNA Complex

Deposited:

2018-09-10

Released:

2019-09-11

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Lou, Y.C., Chou, C.C., Yeh, H.H., Chien, C.Y., Sadotra, S., Hsu, C.H., Chen, C.
Structural basis for -35 element recognition by sigma4chimera proteins and their interactions with PmrA response regulator. 
Proteins, 88, pp. 69 - 81, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

I 4 2 2

Cell Constants:

a = 154.54   b = 154.54   c = 144.96 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2256 for 13125 reflections in the resolution range 29.616 to 3.748 Ångstroms with Fobs >  1.38 sigma(Fobs) and with I >  0.0 sigma(I)

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Asymmetric Unit coordinates (pdb format, Unix compressed(.gz))

Asymmetric Unit coordinates (cif format, Unix compressed(.gz))

Biological Assembly coordinates (pdb format) 1 2 

Structure Factors (cif format)

Coordinates (pdb format, Unix compressed(.gz))

Coordinates (cif format, Unix compressed(.gz))

NMR Restraints (cif format, Unix compressed(.gz))

XML | Complete with coordinates (xml format, GNU compressed(.gz))

XML | Coordinates only (xml format, GNU compressed(.gz))

XML | Header only (xml format, GNU compressed(.gz))