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NDB ID: 6IS7    PDB ID: 6IS7 


Title:

STRUCTURE OF 9N-I DNA POLYMERASE INCORPORATION WITH DA IN THE ACTIVE SITE

Molecular Description:

DNA polymerase/DNA Complex

Deposited:

2018-11-15

Released:

2019-08-28

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

LinWu, S.W., Tu, Y.H., Tsai, T.Y., Maestre-Reyna, M., Liu, M.S., Wu, W.J., Huang, J.Y., Chi, H.W., Chang, W.H., Chiou, C.F., Wang, A.H., Lee, J., Tsai, M.D.
Thermococcus sp. 9°N DNA polymerase exhibits 3'-esterase activity that can be harnessed for DNA sequencing. 
Commun Biol, 2, pp. 224 - 224, 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 21

Cell Constants:

a = 92.951   b = 106.767   c = 255.903 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.22748 for 60118 reflections in the resolution range 46.29 to 2.8 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)