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NDB ID: 6JCY    PDB ID: 6JCY 


Title:

MYCOBACTERIUM TUBERCULOSIS RNA POLYMERASE TRANSCRIPTION INITIATION OPEN COMPLEX WITH A CHIMERIC ECF SIGMA FACTOR SIGH/E

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
Chimera consisting of ECF RNA polymerase sigma factor SigH
ECF RNA polymerase sigma factor SigE and ECF RNA polymerase sigma factor SigH/DNA Complex

Deposited:

2019-01-30

Released:

2019-05-29

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Fang, C., Li, L., Shen, L., Shi, J., Wang, S., Feng, Y., Zhang, Y.
Structures and mechanism of transcription initiation by bacterial ECF factors. 
Nucleic Acids Res., , pp. - , 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 128.943   b = 163.005   c = 129.315 (Ångstroms)

α = 90.0   β = 117.81   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1959 for 78872 reflections in the resolution range 48.766 to 3.106 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)