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NDB ID: 6JE3    PDB ID: 6JE3 


Title:

CRYSTAL STRUCTURE OF NME2CAS9 IN COMPLEX WITH SGRNA AND TARGET DNA (AGGCCC PAM) WITH 5 NT OVERHANG

Molecular Description:

CRISPR-associated endonuclease Cas9/RNA Complex

Deposited:

2019-02-03

Released:

2019-11-06

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sun, W., Yang, J., Cheng, Z., Amrani, N., Liu, C., Wang, K., Ibraheim, R., Edraki, A., Huang, X., Wang, M., Wang, J., Liu, L., Sheng, G., Yang, Y., Lou, J., Sontheimer, E.J., Wang, Y.
Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States. 
Mol.Cell, , pp. - , 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 2 2 21

Cell Constants:

a = 166.388   b = 165.974   c = 178.76 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2278 for 40154 reflections in the resolution range 41.597 to 2.931 Ångstroms with Fobs >  1.39 sigma(Fobs) and with I >  0.0 sigma(I)