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NDB ID: 6JE4    PDB ID: 6JE4 


Title:

CRYSTAL STRUCTURE OF NME1CAS9-SGRNA-DSDNA DIMER MEDIATED BY DOUBLE PROTEIN INHIBITOR ACRIIC3 MONOMERS

Molecular Description:

CRISPR-associated endonuclease Cas9
AcrIIC3/DNA/RNA Complex

Deposited:

2019-02-04

Released:

2019-11-06

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 2 protein sequences

Primary Citation:

Sun, W., Yang, J., Cheng, Z., Amrani, N., Liu, C., Wang, K., Ibraheim, R., Edraki, A., Huang, X., Wang, M., Wang, J., Liu, L., Sheng, G., Yang, Y., Lou, J., Sontheimer, E.J., Wang, Y.
Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States. 
Mol.Cell, , pp. - , 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 129.199   b = 118.01   c = 323.986 (Ångstroms)

α = 90.0   β = 89.92   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2117 for 159906 reflections in the resolution range 46.284 to 3.069 Ångstroms with Fobs >  1.38 sigma(Fobs) and with I >  0.0 sigma(I)