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NDB ID: 6K4Q    PDB ID: 6K4Q 


Title:

CRYSTAL STRUCTURE OF XCAS9 IN COMPLEX WITH SGRNA AND DNA (CGG PAM)

Molecular Description:

CRISPR-associated endonuclease Cas9/RNA Complex

Deposited:

2019-05-26

Released:

2019-09-25

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Chen, W., Zhang, H., Zhang, Y., Wang, Y., Gan, J., Ji, Q.
Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease. 
Plos Biol., 17, pp. e3000496 - e3000496, 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 177.84   b = 69.669   c = 189.513 (Ångstroms)

α = 90.0   β = 109.35   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.20702 for 54217 reflections in the resolution range 49.38 to 2.7 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)