A Portal for Three-dimensional Structural Information about Nucleic Acids
As of 13-Nov-2019 number of released structures: 10455
Search for released structures

NDB ID: 6KC8    PDB ID: 6KC8 


Title:

CRYSTAL STRUCTURE OF WT NME1CAS9 IN COMPLEX WITH SGRNA AND TARGET DNA (ATATGATT PAM) IN POST-CLEAVAGE STATE

Molecular Description:

CRISPR-associated endonuclease Cas9/RNA/DNA Complex

Deposited:

2019-06-27

Released:

2019-11-06

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 4 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Sun, W., Yang, J., Cheng, Z., Amrani, N., Liu, C., Wang, K., Ibraheim, R., Edraki, A., Huang, X., Wang, M., Wang, J., Liu, L., Sheng, G., Yang, Y., Lou, J., Sontheimer, E.J., Wang, Y.
Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States. 
Mol.Cell, , pp. - , 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 21 21 2

Cell Constants:

a = 131.604   b = 159.517   c = 117.963 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the REFMAC program. The R value is 0.2056 for 44188 reflections in the resolution range 49.35 to 2.9 Ångstroms with Fobs >  0.0 sigma(Fobs) and with I >  0.0 sigma(I)