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NDB ID: 6KQD    PDB ID: 6KQD 


Title:

THERMUS THERMOPHILUS INITIAL TRANSCRIPTION COMPLEX COMPRISING SIGMA A AND 5'-OH RNA OF 3 NT

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor SigA/DNA Complex

Deposited:

2019-08-17

Released:

2020-03-11

Structural Keywords:

RH DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 5 protein sequences

Primary Citation:

Li, L., Molodtsov, V., Lin, W., Ebright, R.H., Zhang, Y.
RNA extension drives a stepwise displacement of an initiation-factor structural module in initial transcription. 
Proc.Natl.Acad.Sci.USA, 117, pp. 5801 - 5809, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 1 21 1

Cell Constants:

a = 185.501   b = 104.044   c = 297.441 (Ångstroms)

α = 90.0   β = 98.5   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.207 for 154582 reflections in the resolution range 44.351 to 3.3 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)