## NDB ID: 6LA8 PDB ID: 6LA8

### Title:

349 BP DI-NUCLEOSOME HARBORING COHESIVE DNA TERMINI ASSEMBLED WITH LINKER HISTONE H1.0

### Molecular Description:

Histone H3.1

Histone H4

Histone H2AX

Histone H2B type 1-J/DNA Complex

### Deposited:

2019-11-12

### Released:

2020-10-07

### Structural Keywords:

B DOUBLE HELIX

### Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Chain I

(DC)(DG)(DC)(DT)(DG)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DA) (DT)(DC)(

DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC) (DA)(DA)(DT)(DT)(D

G)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT) (DA)(DG)(DC)(DA)(DC)(DC)(DG

)(DC)(DT)(DT)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT) (DA)(DC)(DG)(DC)(DG)(DC)(DT)(DG)(DT)

(DC)(DT)(DA)(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT) (DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(DC)(DT)(

DA)(DG)(DA)(DA)(DG)(DC)(DG)(DC)(DT) (DT)(DA)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG)(D

G)(DC)(DA)(DC)(DG)(DT)(DG) (DT)(DG)(DA)(DG)(DA)(DC)(DC)(DG)(DG)(DC)(DA)(DC)(DA)(DT)(DG)(DA

)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DG)(DC)(DA)(DT)(DG)(DC)(DT)(DC)(DG)(DA)(DG)(DT)(DA)

(DT)(DG) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DT)(DC)(DC)(DC)(DG)(DG)

(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA)(DT)(DT)(DG)(DG) (DT)(DC)(

DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG)(DC)(DA)(DC)(DC) (DG)(DC)(DT)(DT)(D

A)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC) (DT)(DG)(DT)(DC)(DT)(DA)(DC

)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG) (DC)(DC)(DA)(DC)(DT)(DA)(DG)(DA)(DA)

(DG)(DC)(DG)(DC)(DT)(DT)(DA)(DC)(DT)(DA)(DG) (DT)(DC)(DT)(DC)(DC)(DA)(DG)(DG)(DC)(DA)(DC)(

DG)(DT)(DG)(DT)(DG)(DA)(DG)(DA)(DC) (DC)(DG)(DG)(DC)(DA)(DC)(DA)(DT)(DG)(DA)(DA)(DA)(DA)(D

A)(DA)(DA)(DA)(DA)(DA)(DC) (DC)(DA)(DG)(DC)(DG)(DG)(DT)(DA)(DC)

Chain J

(DC)(DG)(DC)(DT)(DG)(DG)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT)(DG) (DT)(DG)(

DC)(DC)(DG)(DG)(DT)(DC)(DT)(DC)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT) (DG)(DG)(DA)(DG)(D

A)(DC)(DT)(DA)(DG)(DT)(DA)(DA)(DG)(DC)(DG)(DC)(DT)(DT)(DC)(DT) (DA)(DG)(DT)(DG)(DG)(DC)(DG

)(DG)(DT)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DT) (DA)(DG)(DA)(DC)(DA)(DG)(DC)(DG)(DC)

(DG)(DT)(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT) (DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)(

DA)(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC) (DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)(D

G)(DC)(DG)(DG)(DC)(DC)(DT) (DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DG)(DC)(DG)(DT

)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT)(DA)(DC)(DT)(DC)(DG)(DA)(DG)(DC)(DA)(DT)

(DG)(DC) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT)(DG)(DT)(DG)(DC)(DC)(DG)(DG)

(DT)(DC)(DT)(DC)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG)(DA)(DG)(DA)(DC) (DT)(DA)(

DG)(DT)(DA)(DA)(DG)(DC)(DG)(DC)(DT)(DT)(DC)(DT)(DA)(DG)(DT)(DG)(DG)(DC) (DG)(DG)(DT)(DT)(D

A)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG) (DC)(DG)(DC)(DG)(DT)(DA)(DC

)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG) (DG)(DT)(DG)(DC)(DT)(DA)(DG)(DA)(DG)

(DC)(DT)(DG)(DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC) (DC)(DA)(DA)(DT)(DT)(DG)(DA)(DG)(DC)(DG)(DG)(

DC)(DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC) (DC)(DG)(DG)(DG)(DA)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(D

T)(DT)(DT)(DT)(DT)(DT)(DC) (DC)(DA)(DG)(DC)(DG)(DG)(DT)(DA)(DC)

### Protein Sequence:

Click to show/hide 5 protein sequences

Chain A,E,K,O

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFK TDLRFQSSA

VMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Chain C,G,M,Q

MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRII PRHLQLAIR

NDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Chain B,F,L,P

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRK TVTAMDVVY

ALKRQGRTLYGFGG

Chain D,H,N,R

MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASR LAHYNKRST

ITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

Chain S

MTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGV LKQTKGVGA

SGSFRLAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVKKAKKKLAATPKKAKKP KTVKAKPVKASKPKKAKP

VKPKAKSSAKRAGKKK

### Primary Citation:

Adhireksan, Z., Sharma, D., Lee, P.L., Davey, C.A.

Near-atomic resolution structures of interdigitated nucleosome fibres.

*Nat Commun*, **11**, pp. 4747 - 4747, 2020.

### Experimental Information:

X-RAY DIFFRACTION

### Space Group:

P 21 21 21

### Cell Constants:

a = 121.055 b = 184.073 c = 223.367 (Ångstroms)

α = 90.0 β = 90.0 γ = 90.0 (degrees)

### Refinement:

The structure was refined using the REFMAC program.
The R value is 0.2076 for 67687 reflections in
the resolution range 49.32 to 3.4 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)