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NDB ID: 6M0W    PDB ID: 6M0W 


Title:

CRYSTAL STRUCTURE OF STREPTOCOCCUS THERMOPHILUS CAS9 IN COMPLEX WITH THE AGAA PAM

Molecular Description:

CRISPR-associated endonuclease Cas9 1/RNA Complex

Deposited:

2020-02-23

Released:

2020-09-09

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 1 protein sequences

Primary Citation:

Zhang, Y., Zhang, H., Xu, X., Wang, Y., Chen, W., Wang, Y., Wu, Z., Tang, N., Wang, Y., Zhao, S., Gan, J., Ji, Q.
Catalytic-state structure and engineering of Streptococcus thermophilus Cas9
Nat Catal, , pp. - , 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

C 1 2 1

Cell Constants:

a = 321.708   b = 75.333   c = 70.3 (Ångstroms)

α = 90.0   β = 93.47   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2217 for 43301 reflections in the resolution range 48.874 to 2.76 Ångstroms with Fobs >  1.35 sigma(Fobs) and with I >  0.0 sigma(I)