## NDB ID: 6M44 PDB ID: 6M44

### Title:

355 BP DI-NUCLEOSOME HARBORING COHESIVE DNA TERMINI (HIGH CRYOPROTECTANT)

### Molecular Description:

Histone H3.1

Histone H4

Histone H2AX

Histone H2B type 1-J/DNA Complex

### Deposited:

2020-03-05

### Released:

2020-10-07

### Structural Keywords:

B DOUBLE HELIX

### Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Chain I

(DC)(DG)(DC)(DT)(DG)(DA)(DC)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DC)(DG) (DC)(DA)(

DT)(DC)(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC) (DT)(DC)(DA)(DA)(D

T)(DT)(DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT) (DC)(DT)(DA)(DG)(DC)(DA)(DC

)(DC)(DG)(DC)(DT)(DT)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC) (DG)(DT)(DA)(DC)(DG)(DC)(DG)(DC)(DT)

(DG)(DT)(DC)(DT)(DA)(DC)(DC)(DG)(DC)(DG)(DT) (DT)(DT)(DT)(DA)(DA)(DC)(DC)(DG)(DC)(DC)(DA)(

DC)(DT)(DA)(DG)(DA)(DA)(DG)(DC)(DG) (DC)(DT)(DT)(DA)(DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(D

A)(DG)(DG)(DC)(DA)(DC)(DG) (DT)(DG)(DT)(DG)(DA)(DG)(DA)(DC)(DC)(DG)(DG)(DC)(DA)(DC)(DA)(DT

)(DG)(DA)(DA)(DA) (DA)(DA)(DA)(DA)(DA)(DA)(DA)(DT)(DG)(DC)(DA)(DT)(DG)(DC)(DT)(DC)(DG)(DA)

(DG)(DT) (DA)(DT)(DG)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DA)(DT)(DC)(DG)(DC)(DA)(DT)(DC)

(DC)(DC)(DG)(DG)(DT)(DG)(DC)(DC)(DG)(DA)(DG)(DG)(DC)(DC)(DG)(DC)(DT)(DC)(DA)(DA) (DT)(DT)(

DG)(DG)(DT)(DC)(DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)(DT)(DC)(DT)(DA)(DG) (DC)(DA)(DC)(DC)(D

G)(DC)(DT)(DT)(DA)(DA)(DA)(DC)(DG)(DC)(DA)(DC)(DG)(DT)(DA)(DC) (DG)(DC)(DG)(DC)(DT)(DG)(DT

)(DC)(DT)(DA)(DC)(DC)(DG)(DC)(DG)(DT)(DT)(DT)(DT)(DA) (DA)(DC)(DC)(DG)(DC)(DC)(DA)(DC)(DT)

(DA)(DG)(DA)(DA)(DG)(DC)(DG)(DC)(DT)(DT)(DA) (DC)(DT)(DA)(DG)(DT)(DC)(DT)(DC)(DC)(DA)(DG)(

DG)(DC)(DA)(DC)(DG)(DT)(DG)(DT)(DG) (DA)(DG)(DA)(DC)(DC)(DG)(DG)(DC)(DA)(DC)(DA)(DT)(DG)(D

A)(DA)(DA)(DA)(DA)(DA)(DA) (DA)(DA)(DA)(DC)(DG)(DT)(DC)(DA)(DG)(DC)(DG)(DG)(DT)(DA)(DC)

Chain J

(DC)(DG)(DC)(DT)(DG)(DA)(DC)(DG)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA) (DT)(DG)(

DT)(DG)(DC)(DC)(DG)(DG)(DT)(DC)(DT)(DC)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC) (DC)(DT)(DG)(DG)(D

A)(DG)(DA)(DC)(DT)(DA)(DG)(DT)(DA)(DA)(DG)(DC)(DG)(DC)(DT)(DT) (DC)(DT)(DA)(DG)(DT)(DG)(DG

)(DC)(DG)(DG)(DT)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG) (DG)(DT)(DA)(DG)(DA)(DC)(DA)(DG)(DC)

(DG)(DC)(DG)(DT)(DA)(DC)(DG)(DT)(DG)(DC)(DG) (DT)(DT)(DT)(DA)(DA)(DG)(DC)(DG)(DG)(DT)(DG)(

DC)(DT)(DA)(DG)(DA)(DG)(DC)(DT)(DG) (DT)(DC)(DT)(DA)(DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(D

G)(DA)(DG)(DC)(DG)(DG)(DC) (DC)(DT)(DC)(DG)(DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DG)(DC

)(DG)(DA)(DT)(DT) (DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT)(DA)(DC)(DT)(DC)(DG)(DA)(DG)

(DC)(DA) (DT)(DG)(DC)(DA)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DT)(DC)(DA)(DT)(DG)(DT)(DG)

(DC)(DC)(DG)(DG)(DT)(DC)(DT)(DC)(DA)(DC)(DA)(DC)(DG)(DT)(DG)(DC)(DC)(DT)(DG)(DG) (DA)(DG)(

DA)(DC)(DT)(DA)(DG)(DT)(DA)(DA)(DG)(DC)(DG)(DC)(DT)(DT)(DC)(DT)(DA)(DG) (DT)(DG)(DG)(DC)(D

G)(DG)(DT)(DT)(DA)(DA)(DA)(DA)(DC)(DG)(DC)(DG)(DG)(DT)(DA)(DG) (DA)(DC)(DA)(DG)(DC)(DG)(DC

)(DG)(DT)(DA)(DC)(DG)(DT)(DG)(DC)(DG)(DT)(DT)(DT)(DA) (DA)(DG)(DC)(DG)(DG)(DT)(DG)(DC)(DT)

(DA)(DG)(DA)(DG)(DC)(DT)(DG)(DT)(DC)(DT)(DA) (DC)(DG)(DA)(DC)(DC)(DA)(DA)(DT)(DT)(DG)(DA)(

DG)(DC)(DG)(DG)(DC)(DC)(DT)(DC)(DG) (DG)(DC)(DA)(DC)(DC)(DG)(DG)(DG)(DA)(DT)(DG)(DC)(DG)(D

T)(DT)(DT)(DT)(DT)(DT)(DT) (DT)(DT)(DT)(DC)(DG)(DT)(DC)(DA)(DG)(DC)(DG)(DG)(DT)(DA)(DC)

### Protein Sequence:

Click to show/hide 4 protein sequences

Chain A,E,K,O

MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFK TDLRFQSSA

VMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Chain C,G,M,Q

MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRII PRHLQLAIR

NDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK

Chain B,F,L,P

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRK TVTAMDVVY

ALKRQGRTLYGFGG

Chain D,H,N,R

MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASR LAHYNKRST

ITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK

### Primary Citation:

Adhireksan, Z., Sharma, D., Lee, P.L., Davey, C.A.

Near-atomic resolution structures of interdigitated nucleosome fibres.

*Nat Commun*, **11**, pp. 4747 - 4747, 2020.

### Experimental Information:

X-RAY DIFFRACTION

### Space Group:

P 1 21 1

### Cell Constants:

a = 85.038 b = 218.595 c = 115.931 (Ångstroms)

α = 90.0 β = 90.22 γ = 90.0 (degrees)

### Refinement:

The structure was refined using the REFMAC program.
The R value is 0.2093 for 40456 reflections in
the resolution range 48.03 to 3.81 Ångstroms
with Fobs > 0.0 sigma(Fobs) and with I > 0.0 sigma(I)