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NDB ID: 6MPF    PDB ID: 6MPF     NAKB(beta): 6MPF 

Title:

STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT COMPLEXED WITH A 2-THIOCYTIDINE (S2C32) AND INOSINE (I34) MODIFIED ANTICODON STEM LOOP (ASL) OF ESCHERICHIA COLI TRANSFER RNA ARGININE 1 (TRNAARG1) BOUND TO AN MRNA WITH AN CGC-CODON IN THE A-SITE AND PAROMOMYCIN

Molecular Description:

Ribosome

Deposited:

2018-10-05

Released:

2019-04-24

Nucleic Acid Sequence:

Click to show/hide 3 nucleic acid sequences

Protein Sequence:

Click to show/hide 20 protein sequences

Primary Citation:

Vangaveti, S., Cantara, W.A., Spears, J.L., DeMirci, H., Murphy IV, F.V., Ranganathan, S.V., Sarachan, K.L., Agris, P.F.
A Structural Basis for Restricted Codon Recognition Mediated by 2-thiocytidine in tRNA Containing a Wobble Position Inosine. 
J.Mol.Biol., 432, pp. 913 - 929, 2020.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 41 21 2

Cell Constants:

a = 402.33   b = 402.33   c = 176.03 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.1781 for 375418 reflections in the resolution range 49.563 to 3.33 Ångstroms with Fobs >  0.92 sigma(Fobs) and with I >  0.0 sigma(I)

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