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NDB ID: 6N60    PDB ID: 6N60 


Title:

ESCHERICHIA COLI RNA POLYMERASE SIGMA70-HOLOENZYME BOUND TO UPSTREAM FORK PROMOTER DNA AND MICROCIN J25 (MCCJ25)

Molecular Description:

DNA-directed RNA polymerase subunit alpha
DNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
RNA polymerase sigma factor RpoD
Microcin J25/DNA Complex

Deposited:

2018-11-23

Released:

2019-01-09

Structural Keywords:

B DOUBLE HELIX

Nucleic Acid Sequence:

Click to show/hide 2 nucleic acid sequences

Protein Sequence:

Click to show/hide 6 protein sequences

Primary Citation:

Braffman, N.R., Piscotta, F.J., Hauver, J., Campbell, E.A., Link, A.J., Darst, S.A.
Structural mechanism of transcription inhibition by lasso peptides microcin J25 and capistruin. 
Proc. Natl. Acad. Sci. U.S.A., 116, pp. 1273 - 1278, 2019.

Experimental Information:

X-RAY DIFFRACTION

Space Group:

P 41 21 2

Cell Constants:

a = 172.906   b = 172.906   c = 387.263 (Ångstroms)

α = 90.0   β = 90.0   γ = 90.0 (degrees)

Refinement:

The structure was refined using the PHENIX program. The R value is 0.2647 for 63407 reflections in the resolution range 49.551 to 3.68 Ångstroms with Fobs >  1.33 sigma(Fobs) and with I >  0.0 sigma(I)